dihydroethidium dhe  (Beyotime)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Soft fibrin matrix instructs macrophage M2 polarization via FABP4-mediated enhancement of fatty acid β-oxidation to alleviate MASH

doi: 10.1007/s00018-026-06266-2

Figure Lengend Snippet: Macrophages seeded in a soft fibrin matrix alleviated hepatic steatosis, inflammation and oxidative stress in vitro and in vivo. ( A ) Schematic representation of the co-culture of AML12 hepatocytes and BMDMs. ( B ) Oil Red O (ORO) staining in AML12 cells after co-culture with BMDMs seeded in fibrin matrices of different stiffness for 48 h. ( C) ROS detection of AML12 cells after co-culture with BMDMs seeded in different stiffness fibrin matrices for 48 h. ( D ) Serum TG levels ( n = 5), ( E ) Serum total cholesterol (TC) levels ( n = 5), F . Serum alanine aminotransferase (ALT) levels ( n = 5), ( G ) Serum aspartate aminotransferase (AST) levels ( n = 5) and ( H ) Hepatic malondialdehyde ( n = 5). ( I ) Gross images of liver tissues. ( J ) H&E staining( n = 3). ( K ) ORO staining( n = 3) and ( L ) Dihydroethidium (DHE) staining ( n = 3, scale bar, 20 μm). ( M ) Hepatic macrophages are isolated from the liver using gradient centrifugation. The proportions of iNOS+ macrophages and CD206 + macrophages are analyzed via flow cytometry with the respective antibodies( n = 3). Data are presented as mean ± SD. In all scatter plots with bars, each data point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Lipid staining (Beyotime, China) and Dihydroethidium (DHE) staining (MCE, USA) were performed using a modified ORO staining kit according to the manufacturer’s instructions.

Techniques: In Vitro, In Vivo, Co-Culture Assay, Staining, Isolation, Gradient Centrifugation, Flow Cytometry

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Soft fibrin matrix instructs macrophage M2 polarization via FABP4-mediated enhancement of fatty acid β-oxidation to alleviate MASH

doi: 10.1007/s00018-026-06266-2

Figure Lengend Snippet: Macrophage-specific FABP4 knockdown ameliorated HFD-induced MASH. ( A ) Schematic diagram of the MASH model in mice following intravenous tail vein injection of adenovirus. ( B ) Body weight ( n = 5) and ( C ) Fasting blood glucose levels ( n = 5) in different groups. ( D ) Serum TG levels, ( E ) Serum total cholesterol (TC) levels( n = 5), ( F ) Serum alanine aminotransferase (ALT) levels( n = 5), ( G ) Serum aspartate aminotransferase (AST) levels( n = 5) and ( H ) Hepatic malondialdehyde (MDA) ( n = 5). ( I ) Gross images of liver tissues. ( J ) H&E staining( n = 3). ( K ) ORO staining( n = 3) and ( L ) Dihydroethidium (DHE) staining ( n = 3, scale bar, 20 μm). ( M ) Hepatic macrophages are isolated from the liver using gradient centrifugation. The proportions of iNOS+ macrophages and CD206 + macrophages are analyzed via flow cytometry with the respective antibodies. Data are presented as mean ± SD. In all scatter plots with bars, each data point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Lipid staining (Beyotime, China) and Dihydroethidium (DHE) staining (MCE, USA) were performed using a modified ORO staining kit according to the manufacturer’s instructions.

Techniques: Knockdown, Injection, Staining, Isolation, Gradient Centrifugation, Flow Cytometry

Journal: Poultry Science

Article Title: Targeted intestinal delivery of luteolin microcapsules as a precision nutritional strategy to alleviate heat stress and enhance growth performance in broilers

doi: 10.1016/j.psj.2026.106976

Figure Lengend Snippet: Effect of Lut on 42 °C -induced ROS accumulation in cells. (A) Effect of Lut (5, 10, 20, 40, and 100 μM) on cell viability, n = 6. (B) Protective effect of Lut (5, 10, 20, 40, and 100 μM) on 42 °C-induced cytotoxicity, n = 6. (C, D) Effect of Lut on 42 °C-induced ROS generation measured by 10 μM DCFH-DA fluorescent probe. The results were expressed as the mean DCF fluorescence intensity (n = 6). (E, F) Effect of Lut on 42 °C-induced superoxide anion radical generation measured by 10 μM DHE fluorescent probe. The results were expressed as the mean DHE fluorescence intensity (n = 6). The normal control group was incubated at 37 °C for 6 h. The HS group was incubated at 37 °C for 5 h and then at 42 °C for 1 h. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), ** represents the significant difference compared with the control group ( P < 0.01). # represents the significant difference compared with the HS ( P < 0.05). ## represents the significant difference compared with the HS ( P < 0.01).

Article Snippet: 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA), dihydroethidium (DHE), cell counting kit-8 (CCK-8), RIPA lysis buffer, phenylmethanesulfonyl fluoride (PMSF), and BCA protein assay kit were purchased from Beyotime Biotech.

Techniques: Fluorescence, Control, Incubation

Journal: International Journal of Nanomedicine

Article Title: Application of Cerium-Tannic Acid-Formaldehyde Coordination Polymer Colloidal Nanomaterials to Alleviate Lipopolysaccharide-Induced Acute Lung Injury

doi: 10.2147/IJN.S604112

Figure Lengend Snippet: Antioxidant effect of Ce-TA on LPS-induced ALI mice. ( A ) Schematic illustration of the establishment and treatment schedule of LPS-induced ALI mice. ( B ) Quantitative analysis of the relative fluorescence intensity of DHE in lung tissues of mice in different groups. ( C ) Representative DHE fluorescence images of lung tissues of mice in different groups. ( D ) Concentration of MPO in BALF of mice in different groups. Concentrations of ( E ) SOD and ( F ) MDA in lung homogenates of mice in different groups. Data are presented as means ± SD (n = 5 per group). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Lung tissue sections were stained with 2 μg/mL of DAPI (G1012, Servicebio) and dihydroethidium (DHE, D7008, Servicebio) at 37 °C for 30 min. After three washes with PBS, sections were imaged using a fluorescence microscope (Axio Observer, ZEISS), and data were analyzed by ImageJ software.

Techniques: Fluorescence, Concentration Assay